FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2251_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2251_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491640
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT15250.10223646456249498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3950.044.1298981
AGTCCGC2400.042.283974145
CGAAAGC5400.033.5778433
GCGAAAG5200.033.4992452
CGATACA1102.4393247E-432.94855145
CCGACTA5750.032.785444
GTTGACA10650.032.734851
GACGACT2051.1385055E-831.8320164
CAGTGCA10350.030.1547162
ATTCGCT1000.00608080428.994724145
GTTATGT10050.028.1849541
TATACTG5950.026.804545
ACATGGT13100.026.0093355
GTGCAGC12000.025.9814074
CCACCGA7300.025.868381
ACGACTC2559.500218E-825.5861535
GATATAC3151.029548E-925.3272863
CTATGCG3751.0913936E-1125.1355024
TTACGTG1751.2465603E-424.8851532
GATAGCG4151.8189894E-1224.45338145
Produced by FastQC (version 0.11.5)