FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2251_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2251_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334525
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15770.11816938611116314No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15200.11389820348063917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1500.062.9488681
TTACGTG1750.049.7664642
GATATCG657.342916E-444.608669
ACGTGTT2200.039.5469064
AGCGAAA3350.039.0268551
CAAGCGG750.001480294338.6840323
TAGAGCG800.002038365236.244547
AGACGAC1751.0056465E-733.157743
GCGAAAG4100.031.8877941
GGTTATA3200.031.7770751
GCGTTAA1153.1654583E-431.516999
CGAAAGC4150.031.4599063
ATCTATG7450.031.1982041
CCGTACA950.00472700730.5274374
CTAGTAC1204.0481027E-430.2218993
GTTGACA13700.029.689531
GCTACTA2252.8227078E-828.9956329
GCGATTA1555.5074335E-528.060286145
GTACTAG1050.00764055227.6698341
ACATGGT14650.026.7225275
Produced by FastQC (version 0.11.5)