FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2251_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2251_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943286
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT33230.17099901918708826No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC32440.16693374006708225No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC31460.1618907355891001No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG31230.16070717331365533No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG30320.15602438344124334No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA26710.13744760163969688No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT24590.12653824501385796No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT24030.12365652816929676No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG22710.11686390989283102No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT22560.11609202145232353No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT22440.11547451069991756No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA21960.11300446769029367No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG20680.10641768633129658No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA20420.10507974636775029No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG20000.10291845873432938No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC19670.10122030416521294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC3700.056.811707145
ACATGGT16950.046.619275
GTTGACA17000.046.1471521
GCCGACC2200.046.12612145
GTATTAT21050.044.860211
CGAAGCG657.3407433E-444.6123665
CTGCCGC6250.041.75073145
TTATCGC700.001057105441.4268344
GGTTATA5300.039.7458881
ATCTATG11500.039.1619151
CCGACTA3550.036.7590264
GACATGG22050.035.83754
GCTACTA5100.035.5313269
GGTCCTA28650.035.4197129
TGGTCCT30500.033.5089538
TACTGCG3050.033.276445
GTTACGT2206.1118044E-1033.0177421
GTCGTCG900.003633014932.21507145
ATGGTCC31800.032.139097
GCGTTAA2950.031.942069
Produced by FastQC (version 0.11.5)