FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2251_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2251_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852860
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18850.22102103510541005No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC15990.18748680908941678No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT9310.10916211335975425No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA9090.10658255751237013No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG8940.10482376943460826No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT8890.10423750674202097No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC8840.10365124404943366No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA8670.10165795089463686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGCG203.8692504E-4108.7260369
CCGACGC2850.053.409283145
GCCGACC1152.4883775E-950.42367145
ATCTATG4300.048.9707031
GGTGCGA450.00964704948.322688
GGTTATA2350.046.3479161
AGCGAAA1900.045.8600431
TAGCGTA1602.0008883E-1145.3104865
GCGAAAG2100.041.4704972
CCCGATA700.001057699841.419445145
TATACTG2550.039.802155
AGCGTAT1859.640644E-1139.182856
GCGTATG1859.640644E-1139.1805537
GTTGACA7450.038.9861641
ACATGGT7550.037.4486665
CGTTTAT1001.3787298E-436.305871
TCTAGCG2002.237357E-1036.2611473
GGTTCGA1209.576659E-636.2420128
GAATAAC7200.034.288871
CTTATAT3750.032.917321
Produced by FastQC (version 0.11.5)