FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2251_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2251_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988040
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT31150.15668698818937246No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC23370.11755296674111186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC951.895558E-645.8811571
GTAGAAA12300.038.9469532
GCGACTA800.002036737536.251314
TGACGCG800.002039253736.242188145
TATACTG15700.034.628915
GTTACGT2755.456968E-1231.6997091
CCGTACA1402.7266424E-531.1069832
CGAAAGC4700.030.8863662
GCCGACC1952.6144335E-729.73718145
ATACTGG16150.029.1754826
TTATACT16650.029.1752264
CCTTATA15700.029.1256472
TTACGTG3102.7284841E-1128.0966282
GTATTAT24450.027.6319051
GCGAAAG5050.027.3318461
AGCGAAA4300.027.0307581
GGTTATA5400.026.9056191
GTTGACA14100.026.2759111
ACAAGGC15200.026.2279018
ACATGGT14600.025.8183045
Produced by FastQC (version 0.11.5)