FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2251_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2251_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427423
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20530.14382562141705715No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT19730.13822111595511632No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG15610.10935791282612092No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG15510.10865734964337831No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT15360.1076065048692644No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15260.10690594168652179No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA14820.10382346368245432No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT14650.10263250627179189No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA14460.10130143622458093No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG400.00605454854.36788145
GGTCGTT2250.045.1052067
AAGGTCG2300.044.1261985
CGAATAT1505.3842086E-1043.494305145
CTGCCGC4300.040.459816145
GTCGTTT2600.039.033358
GTAGAAA10200.037.7049372
GTTGACA11400.036.317771
GTATTAT15400.035.8461071
AGGTCGT2900.034.9966436
ACATGGT12600.033.9451565
GCCGACC1301.6567586E-533.457157145
AGCGAAA4000.032.685991
TTACGTG1801.2830424E-732.2507672
TATACTG12900.030.3459285
GCGAAAG4350.030.0265732
TATATAG4600.029.9532743
TACTGCG1703.0151568E-629.8500795
GTTACGT2003.2031858E-729.0542141
CGAAAGC4500.029.007383