FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2248_d08.2_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2248_d08.2_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360107
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC63860.27058095247376496No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG57060.24176869946998164No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA54840.2323623462834524No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT49240.20863460851563084No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG44950.19045746654706758No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA35140.14889155449308017No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT25920.109825529096774No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT25900.10974078717617465No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA24250.10274957872672723No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC23880.10118185319563901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG14600.065.543315
GTACTGA16300.059.5932246
GTAGAAA21900.053.678662
GCAGGTA17500.053.491212
AGCGAAA5750.050.5211331
ACTGATC20900.048.556018
GTTACGT2150.047.290131
ACCGTAC1002.7037222E-643.574481
ACAAGGC21800.043.558828
AGGTACT23450.042.3566744
GCGAAAG6950.041.7643742
CGAAGCG700.001057852841.4212539
CAGGTAC23150.040.723673
GTATGGA23650.040.2273181
AGCAGGT23600.039.697091
CGAAAGC7450.038.9366233
TGGATAG23800.038.382894
GACAGTA14800.036.7333767
ATAGTCG800.002038942436.24368
TTATTCG1001.39347E-436.2436145