FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2248_d08.2_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2248_d08.2_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704077
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA40850.23971921456600845No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG40430.23725453720694545No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC38020.22311198378946492No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT35490.20826523684082354No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG33450.19629394681108894No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA28640.16806752277039125No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT22470.1318602387098705No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC18930.11108652954062521No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG18090.10615717482249923No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT17500.10269488996095834No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG17350.10181464804700727No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG17190.10087572333879277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG11100.062.701565
GCAGGTA11150.061.168432
GTACTGA11600.059.3669476
CGTAGAC400.00604643154.3867763
AGCGAAA3650.053.7205921
ACTGATC13700.051.325168
GCCGACC1850.050.939034145
GTTACGT2000.050.83561
GTAGAAA15150.050.7653732
AGCAGGT14050.049.1040341
GACAGTA10100.044.4989177
TTGGACA10300.044.3464554
ACAAGGC15400.043.776538
CCGACGC3150.043.72423145
GCGAAAG4550.043.055312
GACGACT2050.042.4407424
ACAGTAT11500.042.2334028
GTCCGAA700.00104812441.4984471
TTACGTG2450.041.4606672
CAGTATG11250.041.238859
Produced by FastQC (version 0.11.5)