FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2246_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2246_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871826
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC130820.6988897472307789No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG128410.6860146188801737No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT116310.6213718582816993No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT115540.6172582280617963No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT107390.573717856253733No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT106830.5707261251847127No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC103900.5550729608414458No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC103340.5520812297724254No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA95230.5087545530407207No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG94860.5067778735844037No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA89380.47750164812327645No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT88070.47050313437253255No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA85120.4547431224910863No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC84410.45095003488572116No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG65930.35222290960805114No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG64210.3430340213246317No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG61770.3299986216667575No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA61750.32989177412857823No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA60610.3238014644523583No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA59460.31765773100704875No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT56360.30109636258925776No Hit
ATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTT55930.2987991405184029No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA55750.29783751267478925No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG54970.2936704586857966No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG54110.2890760145440869No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT53780.2873130301641285No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC53750.2871527588568596No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG51930.2774296328825436No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT49600.2649818946846555No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT48720.26028060300476646No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC48250.257769685857553No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG47090.2515725286431538No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC46650.24922188280320925No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC45900.24521510012148565No Hit
GATTAAATCAGAACGCAGAAGCGGTCTGATAAACAGAATTTGCCTGGCGG45730.24430689604696162No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG45440.24275760674336183No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT44580.23816316260165207No Hit
GATAAACAGAATTTGCCTGGCGGCCTTAGCGCGGTGGTCCCACCTGACCC44460.2375220773725763No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG41950.22411271133107458No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG41510.22176206549113006No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT41130.2197319622657234No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA41050.21930457211300622No Hit
CTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG41030.21919772457482692No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA40580.21679365496579278No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAG40280.2151909418931033No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT40070.2140690427422207No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA39950.21342795751314492No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT39930.21332110997496564No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG39560.21134443051864862No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT38820.20739107160601464No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC38760.2070705289914768No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG38600.20621574868604242No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA37880.20236923731158774No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT37000.19766794563169868No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC36960.1974542505553401No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG35480.18954753273007213No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA34660.18516678366472097No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA34410.18383118943747975No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC33690.1799846780630251No Hit
CTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCG33600.17950386414121827No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT33350.17816826991397705No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT33320.1780079986067081No Hit
ACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCT33270.17774087976125985No Hit
CTGTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTG33030.1764587093031083No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC32130.17165057008503995No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC32120.1715971463159503No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG32050.17122317993232278No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT31920.17052867093415733No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC31860.17020812831961946No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTC31750.16962046685963333No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG31690.16929992424509543No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA30740.16422466618157883No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG30640.16369042849068235No Hit
TTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCA30630.1636370047215927No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT30290.16182059657254466No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGT30200.16133978265073784No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA30180.16123293511255854No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG29750.15893571304170365No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG29630.15829462781262787No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT29440.15727957619992458No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC28960.15471523528362144No Hit
GTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACA28750.15359333613273884No Hit
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGCTC28070.14996051983464276No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT28040.1498002485273738No Hit
GTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGA27650.14771672153287752No Hit
TCCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTT27480.1468085174583535No Hit
AACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTT26990.14419075277296073No Hit
CGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGG26470.14141271678029904No Hit
GCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAC25870.13820729063492013No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA25620.1368716964076789No Hit
GGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTT25460.13601691610224453No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT25400.13569637348770666No Hit
CCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCG25270.13500186448954124No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC24580.1313156244223555No Hit
AGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATT24510.13094165803872795No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT24340.13003345396420393No Hit
GCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGG23860.12746911304790082No Hit
AAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGC23650.1263472138970182No Hit
CCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGG23610.1261335188206596No Hit
GCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGC23220.12404999182616333No Hit
GCCTGATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAA22710.12132537960259125No Hit
CTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCA22630.12089798944987407No Hit
CCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG22480.12009663291352936No Hit
GAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGA22150.11833364853357096No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC22010.11758571576631588No Hit
GCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAG21660.1157158838481782No Hit
AAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATA21540.11507479861910241No Hit
TCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTT21460.11464740846638523No Hit
GTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACT21310.11384605193004049No Hit
CCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTG21230.1134186617773233No Hit
AATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATG21230.1134186617773233No Hit
CCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT21020.1122967626264407No Hit
GTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTC20890.11160225362827528No Hit
GTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAA20870.11149540609009599No Hit
ATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATT20450.10925160778833076No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGAC20290.10839682748289639No Hit
CTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGG20260.10823655617562744No Hit
GGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCG20150.1076488947156413No Hit
TAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAG20070.1072215045629241No Hit
CCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTC19960.106633843102938No Hit
GTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTC19930.10647357179566905No Hit
GGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCA19910.10636672425748973No Hit
GGTTAATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAAC19590.104657163646621No Hit
GTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCC19550.10444346857026242No Hit
AGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGT19540.10439004480117275No Hit
ATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCG19480.10406950218663487No Hit
GCATATGGATGCGCTGTTTATCGATCCTGATGTGCGCGGCTGCGGCGTGG19010.1015585850394214No Hit
GCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTG18810.10049010965762845No Hit
GGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCG18780.1003298383503595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGTG24400.071.904984
GGAGCAA22950.070.581651
GTCATAG24900.070.5421452
CAAACGC23200.069.6830445
ATAAGAC38100.068.334843
CGCGCCA62200.066.662429
ACGCGCC62300.066.555428
TAAGACG38950.065.519214
TCATAGT26850.065.094673
ATAGTGA28250.061.588985
GATAAGA43700.059.6273732
AAGACGC42750.059.352935
AGTGATC30100.058.76237
TAGTGAT30100.057.8020676
AGACGCG44600.056.726926
GACGCGC45100.056.2557537
AACGCGC28650.055.9168977
GGATAAG51850.054.4967351
AAACGCG32900.049.577576
TGATCCG38050.047.627839
Produced by FastQC (version 0.11.5)