FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2246_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2246_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064035
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG8200.038.0639422
GTATAGG4300.035.474451
CGAAAGC9550.032.6546633
AGCGAAA9350.032.6289061
CGCGCCA4000.032.6206559
TGATCCG3800.032.4298939
ACGCGCC4450.029.3219388
ACCGTGC2054.0473788E-728.2889148
GCCGACC3503.6379788E-1226.924986145
AACGCGC1658.430463E-526.3601267
GACGCGC3501.3096724E-1024.8538347
GTATAAG6750.022.5985371
TATACTG13050.022.220345
AAACGCG2053.6709665E-421.21726
GTATATA9450.020.7537571
TTATACT10500.020.715044
GTCCTAT17800.019.9958971
TCTATAC24950.019.1846333
TTATAGT12550.019.064444
AGTGCGT4253.2818207E-818.7622098
Produced by FastQC (version 0.11.5)