FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2246_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2246_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777390
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG7300.028.842172
GAATAAC8450.028.3809091
AGCGAAA7850.026.8471011
CGATACC1100.00967054726.3653264
TCTATAC16600.025.3422183
GTCCTAT15250.024.7800711
CGAAAGC8850.024.5868383
TAGGACT13700.023.8153934
ATAGGAC8000.023.5726343
GGTACTG8950.022.6798255
TATAATA8650.022.6621152
CTACGGG1952.614719E-422.3091224
GTACTGA12150.020.8819986
TCCTATG18450.020.8560622
TATACCA18850.020.767645
GCCGACC3851.05483196E-820.710367145
GTATATA5650.020.5797751
GTTCTAT20300.020.4055291
GGATAGC8700.019.9984685
CTATACC19600.019.6058464
Produced by FastQC (version 0.11.5)