FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2246_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2246_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307136
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC29260.1268239063496907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA4550.049.3888789
ACGCGCC4750.047.3093458
GACGCGC3350.041.1138047
AACGCGC2200.039.540077
TGATCCG4750.035.1004839
GCGAAAG10450.033.3308532
AGCGAAA10500.033.197341
CGAAAGC11200.031.7302473
AGACGCG5350.027.0991136
ATAAGAC11300.025.673023
TTATTCG2859.924406E-925.435133145
AAACGCG3601.8553692E-1024.1633766
CTGCCGC9750.023.791632145
TAGTACC15950.022.2721084
GTACCTA13150.022.0502286
GTCCTAT24150.021.951141
TACCTAT13000.021.1894237
TCTATAC33250.020.9399193
TATTATA14300.020.2976342
TTATACG2908.2591505E-619.9994434
Produced by FastQC (version 0.11.5)