FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2246_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2246_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748293
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18080.10341515981588899No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA3000.045.9101569
ACGCGCC3300.041.7365048
GACGCGC2350.040.101847
AACGCGC1354.978647E-737.5883377
GCGAAAG5700.034.3726232
AGCGAAA5850.033.5325551
GCGTTAA950.004731534530.5219849
ATCGATA950.004731534530.5219848
CGAAAGC6950.029.2153533
AGACGCG3600.028.1920596
GTGATCG2859.920768E-925.4349889
GCACCGT6600.025.2630146
AAACGCG2101.5569534E-524.1646236
TATACTG10150.023.5707845
ACCGTGT7650.022.7418738
AGCACCG7450.022.3819375
GGATAGC3304.6964487E-821.9690935
GTCCTAT17950.021.8568741
GCCGACC2404.3700573E-521.142834145
CACCGTG9650.021.0338887
Produced by FastQC (version 0.11.5)