FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2246_d08.2_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2246_d08.2_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361875
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG1307.357812E-944.612679
GTCTACG855.3317104E-542.6616943
GCGAAAG4050.041.2081642
CGAAAGC4450.039.1145443
AGCGAAA4100.038.973051
ACCGTAC951.0181561E-438.2271541
GTTACGT2201.4551915E-1136.3157961
CCGACGC1601.8786686E-631.71682145
ACGACTC1851.644712E-731.3505955
TACTGCG1753.776644E-628.9993025
GCGATTA1255.1566405E-428.998238145
TTACGTG2802.6011548E-1028.5065782
GCGTTAA1958.761659E-626.024069
TAGACGT1409.995716E-425.8931854
TGCGTTA2051.29131895E-524.7545958
CCGTACA1500.001487104824.1873992
GTGATCG1500.001495079324.16529
GTATACC2751.9631989E-723.7354163
GATAGCG2751.9708568E-723.72583145
CTACTGA16050.023.487676
Produced by FastQC (version 0.11.5)