FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2246_d08.2_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2246_d08.2_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489484
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC657.2772685E-444.6904031
AGCGAAA4000.038.12651
GTTACGT2103.7107384E-1034.581861
GCGAAAG4550.033.5178031
TATACTG8100.031.3240055
CGAAAGC5150.029.582972
GATAGCG2603.7271093E-927.88181145
CTACGCA1100.009641455526.381352
GACGCGC1400.001000277325.8902477
TTACGTG2859.833457E-925.4556882
GTATACG2401.575745E-624.2073021
GGTTATA5450.022.6527041
GTTGACA13200.022.0066381
GTATATA8300.021.8740671
GTGCTAC4006.7666406E-1021.7865721
TATAGTA4701.0913936E-1121.6102562
AGTCCGC1700.003095303721.321383145
ACGTAGC1700.003095303721.321383145
CGCGCCA2053.6700987E-421.2173779
CACGACG2404.368114E-521.1437055
Produced by FastQC (version 0.11.5)