FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2244_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2244_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801125
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6850.044.5423051
GCGAAAG7100.042.9368822
CGAAAGC7300.041.728053
GATAGCG3700.031.34663145
GTGATCG1402.7507189E-531.0667489
GCGATTA1255.1600096E-428.995628145
TATACTG10250.026.1711485
GTTACGT3201.2132659E-924.9721951
CCGACGC3201.2441888E-924.918121145
TTACGTG3452.930392E-923.1426542
GTAGTAC3055.325728E-721.4015083
TAGACAG13350.021.180115
GTATATC6900.020.0040761
CCGACTA4402.2646418E-919.7741374
GTATAGG5707.2759576E-1219.1174681
CCTAGAC6650.018.5408233
CCTATAC6401.8189894E-1218.1318343
ACGAACC5209.1677066E-1018.1252865
TAGTACC12500.017.9812854
GTATTAG6152.3646862E-1117.718631
Produced by FastQC (version 0.11.5)