FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2244_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2244_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231334
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC3250.064.68198145
GCCGACC2850.045.782166145
CTGCCGC8250.036.02455145
GCGAAAG4450.030.9891262
ACGACGC1255.1593425E-428.99537145
AGCGAAA4850.028.4529881
CGTACAC1907.1389877E-626.7192843
CGAAAGC5350.025.7561043
GCGTAAT1751.2407705E-424.9017121
GGCCGTC1902.1959412E-422.8910837
GCACCGT4557.2759576E-1222.3050386
ACCGTGT4607.2759576E-1222.0616958
CCGTACA1750.003635954320.7370832
TAGGGTG5555.456968E-1219.594655
CCGTGTT5950.019.4926859
GTACATA5605.456968E-1219.4544621
GTGTAGG5707.2759576E-1219.1131571
CATGTAC4603.918103E-918.9192433
GGCGCGC1950.006847740618.5867778
GTACCGG2400.001047230218.15751
Produced by FastQC (version 0.11.5)