FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2244_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2244_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908074
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC2252.0008883E-1135.436615145
TACGGGG1153.1672677E-431.514787
GACGAAC2054.0502164E-728.286436145
GCGAAAG5950.028.046952
AGCGAAA6200.025.7707751
CGAAAGC6400.024.9412082
AGTCGAA2101.5467143E-524.1854132
GTATTAT22950.023.1013131
GGTTATA7850.022.204351
GTTACGT2455.048758E-520.7504941
AAGTCGA2455.048758E-520.7504941
ACTCCGA6300.020.7504921
GACGACT2104.314147E-420.7156834
CCGAGGT7350.020.7156814
TAGTACC12050.020.4578114
GCACCGT5700.020.3485166
GTACCGA1800.00426514320.1740911
GCTATAT4702.3101165E-1020.0882421
CATCGTT4001.6194463E-819.9529652
GATATAT11850.019.612281
Produced by FastQC (version 0.11.5)