FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2244_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2244_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658737
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC19240.11599186610053312No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG400.00605463254.3680849
GTCTACG951.0186716E-438.224481
CGAAGCG800.002038273336.2453887
GTTACGT2151.7815182E-830.4017931
ACTCCGA5600.025.9380381
GCGAAAG4650.024.9897651
AGCGAAA4650.024.9897651
GGTGCGA1751.2572992E-424.853988
GCCGACC2051.2922053E-524.752949145
GCGTTAA2101.5574053E-524.1635919
AGTCGAA2252.6308735E-522.5799142
GTATATA8100.022.415591
CGAAAGC5200.022.3317812
TACTGCG2653.7845984E-621.8853285
CCGAGGT6750.021.4826354
GTAGCCG4058.076313E-1021.478759
GGTTATA5850.021.1051391
TATTGGG11700.021.0911272
TAGTCGG2806.1009705E-620.711658
CGAGGTG7100.020.4211675
Produced by FastQC (version 0.11.5)