FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2244_d08.2_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2244_d08.2_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659227
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10330.15669867890726563No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC8850.1342481421422363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCC1009.094947E-1265.2390448
CATAGCG400.00605399654.3658687
CGCGCCA1301.3096724E-1050.183889
AACGCGC604.9479434E-448.3252147
GACGCGC907.487068E-540.2710157
TAAGACG1251.2645751E-534.7994354
TAGCATG9750.033.501662
GCATGTT9850.033.1212924
CATGTTG9150.032.4833455
GTAGCAT12350.032.355651
AAACGCG900.003628976432.2192576
GATATAC1204.0497378E-430.217013
GTTACGT1000.0060106829.061261
GTCTACA1255.0887075E-429.0612581
CGTATGC1000.00607796128.9951298
AGCATGT11300.028.8799783
GTTGTTT14100.027.7612958
TATACTG2903.947207E-1027.4974675
ATATATC2654.5383786E-927.366353
CCTATAC1100.00965570726.371213
Produced by FastQC (version 0.11.5)