FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2244_d08.2_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2244_d08.2_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823071
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18560.10180623793587852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC1750.049.704847
CGCGCCA2500.049.2879379
ACGCGCC2500.049.2879378
GCGAAAG5850.038.4568632
AGCGAAA5950.037.8385851
CGAAGCG800.00203950336.2411279
CGAAAGC6350.035.4102863
CCGACGC2950.034.398357145
CTGCCGC6750.028.992903145
GTTGACA15600.027.001851
AACGCGC1400.001000884325.887947
ACTCCGA4950.024.9421481
TAAGACG3803.6379788E-1022.8947854
GTTACGT3604.7584763E-922.1911771
AAACGCG1650.002604128821.9661276
TATAGTA5650.021.835772
AGACGCG4006.9303496E-1021.7464686
CCGACTA4058.076313E-1021.4815254
ACATGGT17950.021.4043795
TTACGTG3757.68523E-921.2877352
Produced by FastQC (version 0.11.5)