FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2243_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2243_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473173
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC337262.2893441571356523No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG335032.2742067632246856No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG226251.5358006154063373No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA98610.6693714859015201No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA84330.5724378603191885No Hit
GAAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCT77070.5231564792458183No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG76560.5196945640464493No Hit
GAATCGAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGA73030.4957326804116013No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC61270.41590498875556364No Hit
AAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTATATTC60090.4078950673138865No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT57690.3916037016697971No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA56830.38576596231399846No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA53840.3654696359490705No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG52440.3559663393233517No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA51690.35087528755957376No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG50510.34286536611789653No Hit
AAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAAAA49250.33431239915474964No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA47240.3206683804278248No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG46570.3161203741855166No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT46270.3140839534800054No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT45640.30980746999843195No Hit
GGATAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG44010.29874291749848797No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA43650.2962992126518746No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG43560.2956882864402212No Hit
GCCTAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAAC43310.29399126918562857No Hit
CCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAAC42940.2914796836488315No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA42470.2882892912101973No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC40180.27274461315812876No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA37780.2564532475140394No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA37520.25468834956926306No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT36830.25000458194658737No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG35680.24219830257546127No Hit
AAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA35400.2402976432503175No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT35010.237650296333153No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA34360.23323805147121213No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT33670.22855428384853646No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA33470.22719667004486235No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA33220.22549965279026973No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC32730.22217349897126815No Hit
CTCTCTGGGTCCTCTGTAAGCGCACCTGCGTCCTTCCCAAGTATTGTAAG31150.21144834992224268No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG30610.20778279265232258No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG30430.20656094022901586No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG30230.20520332642534178No Hit
GTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAAT29430.1997728712106453No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG28790.19542850703888817No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA28720.19495334220760224No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA28710.19488546151741853No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACTAG28630.19434241599594887No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT28600.19413877392539777No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT27970.18986229044382433No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT27240.18490700006041383No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT26660.1809699200297589No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA25980.17635403309726694No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC25950.1761503910267158No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG25920.1759467489561647No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG24730.16786894682430373No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC24680.16752954337338521No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA24490.16623981025989482No Hit
CCTTCCCGATTGAGCCTTCCTCCACTCCCTTAGGTGACTCTCCAATTGGC24350.1652894805973229No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC24150.1639318667936488No Hit
GGGTACTCCCCTGCATTTAACTACAACAAGACAACTAAGAGGCTTACAAT23340.15843353088876866No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG23030.1563292294930738No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC22360.1517812232507655No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG22340.1516454618703981No Hit
CCCATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAGA22200.15069513220782624No Hit
ATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAAT22180.15055937082745882No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGCTGTTGAACAC22100.15001632530598918No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA22030.14954116047470326No Hit
CAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAA21990.14926963771396842No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA21910.14872659219249879No Hit
GTTTTCTAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCTGCACACCT21520.14607924527533428No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACT21370.14506103492257866No Hit
AGTCTGCTTCATGTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGA20760.14092031282137266No Hit
GTTCATTGATGCTTAATGCTGGTCTTTTGTCTTCTTTGCCGAGGATTAGA19860.13481105070483915No Hit
GTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAAAACTACT19800.13440376656373693No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG19630.13324979483061392No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT19150.12999152170179604No Hit
GCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCA18860.1280229816864686No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTAC18770.12741205547481524No Hit
GGAGTACCCTGTCTGATCCAGCGTCATTTGTTTTGCTCCAAAGAGTTTGG18110.1229319299226907No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA18030.12238888440122105No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT17860.12123491266809805No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT17780.1206918671466284No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG17590.119402134033138No Hit
CTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATG17560.11919849196258689No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA17530.11899484989203576No Hit
CATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAATGAAATGGATGATG17440.1183839236803824No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAG16820.11417532088899268No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC16660.11308922984605338No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG16600.11268194570495116No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG16570.11247830363440003No Hit
GTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCA16410.11139221259146073No Hit
CTCTACTCCTGCTGTTCCTTCATCTGGGTCCTCTGTAAGCGCACCTGCGT16230.11017036016815404No Hit
CTGTAAGCGCACCTGCGTCCTTCCCAAGTATTGTAAGCCTCTTAGTTGTC16180.10983095671723551No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT15770.10704784841970359No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA15560.10562235392584578No Hit
GGATACACCGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATG15350.10419685943198796No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGC15310.10392533667125314No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATAGC14950.10148163182463973No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT14940.10141375113445603No Hit
CCCAGAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGA14930.10134587044427232No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC14770.10025977940133304No Hit
GTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATT14760.10019189871114934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC100.0070835548145.052343
GCAGGTA44050.0115.365172
AGCAGGT46750.0109.090821
GGTACTG46650.0108.939195
CAGGTAC47900.0106.290963
GTACTGA51450.099.050866
ACTGATC53350.097.1505368
TACTGAT55150.092.79997
AGCGAAA4200.089.947021
AGGTACT64400.081.280034
GTTACGT502.278719E-672.649521
TTGGACA47850.071.968794
GACAGTA48250.071.06447
GCGAAAG5250.070.523442
TTTTTGG49350.068.895591
TTTGGAC50700.068.234673
ACAGTAT51400.066.707028
CAGTATG56350.060.8472259
TGGACAG58250.060.2375375
GGACAGT59250.059.4615486
Produced by FastQC (version 0.11.5)