FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2243_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2243_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715853
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG349512.036946055402182No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC323031.882620480892011No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG243931.4216252790885933No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA123830.7216818690179171No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG100270.5843740693404389No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA89100.5192752526003102No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG78230.4559248373840883No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC73560.4287080536619396No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA68380.39851898734914937No Hit
GAAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCT68010.3963626254696644No Hit
GAATCGAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGA67520.39350690298061664No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA63940.3726426447953292No Hit
GCCTAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAAC63170.3681550808839685No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT63130.3679219606807809No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG62930.3667563596648431No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA58960.34361917949847687No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG57660.3360427728948808No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT55940.32601860415781536No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT55220.3218224405004392No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA54990.32048199933211063No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC54350.3167520760811095No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT51200.29839386008008845No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA50810.29612093809900963No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG50200.2925658550003992No Hit
GGATAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG47130.2746738794057533No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA45870.2673305930053449No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG45100.26284302909398416No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT44570.25975418640174885No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA44200.2575978245222639No Hit
AAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAAAA43480.25340166086488763No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG42100.24535901385491646No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA41720.2431443719246346No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA41110.23958928882602415No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA40680.23708324664175776No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACTAG40470.23585936557502304No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG39890.23247912262880327No Hit
CCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAAC38480.22426163546644148No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG38230.2228046341965192No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT37860.22064827231703413No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG37470.21837535033595534No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA37040.21586930815168898No Hit
AAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTATATTC36530.21289702556104745No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGCTGTTGAACAC35660.20782666114171786No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG34330.20007541438573118No Hit
GTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAAT34160.19908465352218402No Hit
AAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA33860.19733625199827723No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC33320.19418912925524506No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT33300.19407256915365126No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACT32810.19121684666460356No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT32520.1895267251914937No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC31730.1849226011785392No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC31080.18113439787674118No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA30500.17775415493052144No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG30360.17693823421936494No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT30310.17664683396538047No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA29650.1728003506127856No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA29430.17151818949525396No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT29310.17081882888569125No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC28470.1659233046187523No Hit
GTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAAAACTACT28290.16487426370440825No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG28200.16434974324723622No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG28060.1635338225360797No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT27380.15957077908189105No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTAC27240.15875485837073455No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG26770.1560156959832806No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA26640.15525805532292103No Hit
GGGCTATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTT25810.15042081110677896No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA24760.14430140577310527No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT23710.13818200043943157No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA23050.1343355170868367No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGC22490.13107183424221072No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA21810.12710879078802206No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA21590.1258266296704904No Hit
GCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCA21470.12512726906092772No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA21370.12454446855295878No Hit
GGGTACTCCCCTGCATTTAACTACAACAAGACAACTAAGAGGCTTACAAT21200.12355370768941162No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATAGC20070.11696806194936281No Hit
AGTCTGCTTCATGTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGA20050.116851501847769No Hit
GTTCATTGATGCTTAATGCTGGTCTTTTGTCTTCTTTGCCGAGGATTAGA19940.11621042128900319No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC19930.1161521412382063No Hit
GTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCA19850.11568590083183117No Hit
GTAGTTTTCTAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCTGCACA19550.11393749930792439No Hit
CTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATG19500.11364609905393994No Hit
GTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATT19330.11265533819039278No Hit
CAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAA19250.11218909778401762No Hit
GGAGTACCCTGTCTGATCCAGCGTCATTTGTTTTGCTCCAAAGAGTTTGG19140.11154801722525182No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG19090.11125661697126736No Hit
ACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAG18860.10991617580293883No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAG18610.10845917453301653No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG18520.10793465407584449No Hit
ACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGC18510.10787637402504761No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG18350.10694389321229733No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA18240.10630281265353152No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG18110.1055451719931719No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC18050.10519549168839055No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA17880.10420473082484338No Hit
GGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAAAACT17670.10298084975810864No Hit
ATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAAT17530.10216492904695217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA47750.0110.343592
AGCAGGT48250.0109.446341
GGTACTG48700.0108.2327655
CAGGTAC50550.0104.43953
GTACTGA53900.097.773916
ACTGATC56150.094.366898
TACTGAT60350.087.439197
AGCGAAA4300.079.395071
AGGTACT71350.075.807254
TTGGACA56750.067.330324
TTTTTGG57100.066.78631
GACAGTA57350.066.482047
TTTGGAC58650.065.0387653
ACAGTAT58650.064.761268
CTGATCC86850.060.926349
ACAAGGC16000.059.3476228
GGACAGT66800.058.925156
CAGTATG64400.058.8664479
TAACCGG502.0126201E-457.9885189
AGTAGAA33400.057.849471
Produced by FastQC (version 0.11.5)