FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2243_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2243_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516959
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG158071.042018933932954No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC150220.9902706665110922No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG93190.6143211517252609No Hit
GAAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCT79260.5224926975613712No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA48200.3177409541062085No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA47960.3161588414716548No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG43440.2863623868542261No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC36980.2437771884408214No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG31800.20962992407836997No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA31740.20923439591973153No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG30900.20369700169879346No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT30520.2011919900274167No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA30340.20000540555150137No Hit
GAATCGAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGA29610.1951931462880671No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC29470.1942702472512441No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA28140.1855027064014255No Hit
GCCTAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAAC27710.18266808793118336No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT27520.18141558209549502No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA27410.1806904471379912No Hit
AAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA27350.18029491897935276No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG27010.1780535927470683No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC25310.16684696158564602No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA23840.15715652169900438No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC22900.15095991388033558No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG22810.15036662164237793No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA22730.14983925076419335No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC22590.14891635172737033No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT22370.14746608181236276No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG21850.144038171104163No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA21530.14192868759142468No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC21370.14087394583505553No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT21060.1388303836820903No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC20810.1371823496877635No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC20640.13606168657162127No Hit
GAGAGCATGATCGAGGCTGAGTCTTCTATCAAAGAGAAAGACATGACCAA20630.13599576521184817No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG20130.13269969722319455No Hit
CCTTCCCGATTGAGCCTTCCTCCACTCCCTTAGGTGACTCTCCAATTGGC20040.1321064049852369No Hit
CCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAAC19830.1307220564300024No Hit
GGAGTACCCTGTCTGATCCAGCGTCATTTGTTTTGCTCCAAAGAGTTTGG19580.1290740224356756No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG19040.12551426900792967No Hit
GTTTTCTAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCTGCACACCT18910.12465729133087973No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA18430.12149306606177228No Hit
GGGTACTCCCCTGCATTTAACTACAACAAGACAACTAAGAGGCTTACAAT18320.12076793110426848No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT18010.11872436895130323No Hit
CTCTCTGGGTCCTCTGTAAGCGCACCTGCGTCCTTCCCAAGTATTGTAAG17860.11773554855470715No Hit
ATTGTGGAATCTGGTGATCCAAATGCATTGTTGAAGCACAGGTTTGAAAT17770.11714225631674949No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC17650.11635119999947262No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG17480.11523053688333039No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG17100.11272552521195366No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT16920.11153894073603836No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC16910.11147301937626528No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16890.11134117665671914No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT16870.11120933393717299No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA16850.11107749121762685No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT16700.11008867082103076No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA16490.10870432226579624No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG16480.10863840090602317No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT16000.1054741756369157No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG15900.10481496203918499No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG15900.10481496203918499No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACTAG15620.10296916396553896No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA15620.10296916396553896No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG15460.1019144222091698No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGCTGTTGAACAC15310.1009256018125737No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA15250.10053007365393526No Hit
CTCTACTCCTGCTGTTCCTTCATCTGGGTCCTCTGTAAGCGCACCTGCGT15240.1004641522941622No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA15200.1002004668550699No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC151.2289282E-4145.035583
GGTACTG20250.0103.095615
GCAGGTA20500.0102.997632
CAGGTAC22450.093.352523
TGTACGA259.357675E-487.070162
GTACTGA24150.087.046966
AGGTACT29650.073.349634
TTGCCGC801.0004442E-1072.48672145
CTGCCGC1550.065.47188145
ACTGATC39650.063.9857528
GACAGTA23650.062.832047
GCGAAAG3600.062.48092
TATAGCG2700.061.809062
AGCAGGT35750.059.3271751
GCGGTAT3100.058.4589586
TTTGGAC25500.057.7298473
CGAAAGC3900.057.6423453
TTTTTGG25850.056.7593841
TACTGAT38800.056.233257
GGTCAAA3250.055.8731961
Produced by FastQC (version 0.11.5)