FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083633
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG9050.054.546842
CGAAAGC9500.051.9156273
AGCGAAA10500.047.0616841
TATACCG3800.036.241979
GCCGACC3200.031.711721145
GTATAAT12550.030.1097721
TAACCCG1753.7787777E-628.9984465
TATAATA15500.028.1014542
ACGATTA2808.223651E-925.887747
CGATTAT2808.227289E-925.8871178
GCACCGT7650.025.5844046
GTATATA12800.024.9798931
ACCGTGT6900.023.1108198
AACGATT3503.5124685E-922.7823056
TATACTG11250.022.5543465
GTCCTAT17450.022.071321
GTATTAT19250.021.5175081
TCGCCAT2806.1071933E-620.709694145
TAGACAG18300.020.5999895
CTAGGAC11800.019.6689323
Produced by FastQC (version 0.11.5)