FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533840
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4800.057.495481
GCGAAAG4800.055.9440732
ACCGTAC1351.8735591E-1048.4172481
CGAAAGC7050.038.0647353
TCGTATA850.002744123834.113377145
CCGTACA1956.9267116E-933.496662
ATATACG1000.006075329.000154
TATTGCG1000.00607917228.996377
GACGTAT1100.00967906926.3603347
GTCCTAT13300.024.0266041
GCCGACC2252.6553022E-522.552732145
ATAGTCC5600.022.0176563
AACGTGC3406.445589E-821.320868
TAACGTC2053.6707774E-421.2168529
TAGGTAG4251.4733814E-920.469365
TACACCG2908.258487E-619.99885
TCTATAC14950.019.8908273
ATACCGC3301.1401589E-619.770256
CCTATAC6000.019.3413
GCACCGT4503.0013325E-919.3309146
Produced by FastQC (version 0.11.5)