FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153984
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG450.00962398348.354493
GTCCTAT16150.023.836761
AGCGAAA11650.023.6919881
CTGCCGC7450.023.349655145
GTATACG2953.8099643E-722.1597251
ACCGTAC1750.00362093820.7527581
GTATAGT6000.020.5798191
AACGAGC2857.120143E-620.3456068
CACCGTG10050.020.1937737
GCGAAAG14200.019.9336512
GCCGACC3001.1089693E-519.328327145
TCTATAC18900.019.188293
ACGCGTG3401.520244E-619.1862057
CGAAAGC15000.018.858253
TACACCG5551.10958354E-1018.2882215
TAGACAG18850.018.0768575
CCTATAC6152.5465852E-1117.6906663
TATACAT13600.017.5918465
CCGTACA2901.8511641E-417.5190542
GTATAGA18050.017.3035471
Produced by FastQC (version 0.11.5)