FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560645
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5850.040.937442
AGCGAAA6050.039.6044581
ACCGTAC1406.536029E-736.304091
CGAAAGC7150.033.470653
GTACGAA1153.1353685E-431.5687711
GTGATCG1402.7497625E-531.067949
TAGCGAG1100.0096785126.3606765
CTTCGAC1851.8279301E-423.5199183
CAGCGTA3102.4228939E-823.3844729
CGAAAGT1600.002175896422.6558844
CTGCCGC6400.022.653708145
GTATAAG4151.0732037E-920.9951341
TACGCCG2455.1207848E-520.711965
GTAGTGT6350.020.5488746
AGTGATC16550.020.1487968
CCGTAGA2205.8463827E-419.8022291
TACGAAA1900.00585616419.097612
GTATATA9150.019.044771
GTATTAT13850.018.8728831
TATACAG7300.018.8677445
Produced by FastQC (version 0.11.5)