FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197542
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA8700.055.9328351
GCGAAAG9150.053.931542
CGAAAGC14150.035.3647653
TGATCCG4800.034.7341889
CATAGTG6750.027.92544
CTGCCGC10300.027.44719145
ACGCGCC4350.026.6625238
AGACGCG2559.502946E-825.586596
GCCGACC3709.094947E-1225.469017145
CGCGCCA4450.024.4344069
GTATTAG5400.024.2097361
GTATATA12050.024.109281
ATAATCG2003.1055472E-421.7490965
TAACGAC2704.4364424E-621.4884133
ATTACGA2806.0991333E-620.7129546
GTATAGA16500.019.3677881
CGACCCG3451.7465427E-618.910105145
ACCGTGT6600.018.6713438
CGTATGC2751.2445393E-418.451688
TATACTA6351.8189894E-1218.2669565
Produced by FastQC (version 0.11.5)