FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520573
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5050.053.2188951
CGACGAA701.6983295E-551.775803145
GCGAAAG6250.042.9626462
CGAAAGC7500.034.808223
TAGCGTA1358.0937025E-426.846712145
GTCCTAT12000.026.6334191
ATTGCGA1100.00968216726.35859145
GTATAAA8750.022.4135781
ATTATAC7250.022.0051983
TATGATG13550.021.9366765
TGGATCG1750.003660166420.7137265
TCCTATG16000.020.4108852
GCACCGT5353.6379788E-1220.3238816
CTATGAT14450.019.569524
CTGCCGC11150.019.502993145
TATACTG8550.019.5023965
TAGTACT5950.019.4978374
CAGCGCG3351.3177469E-619.4738859
CGATACA1900.005895782719.075294145
GGATCGC1950.006848418618.5867986
Produced by FastQC (version 0.11.5)