FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525633
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC27610.18097406125850712No Hit
ATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTCATGAAGCTTA21250.13928644700265397No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC20930.13718895697720226No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT19140.12545612214733165No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC19130.12539057558403627No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC18330.12014685052040694No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC18100.1186392795646135No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT18060.11837709331143204No Hit
GTTTATGATGTTGGTATTGAATACAACATTAATCATCCCTGTGGTTATAA15380.1008106143482738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1450.089.981384145
CCGACGC5200.062.72741145
GCGAAAG4450.057.087392
AGCGAAA5050.048.907581
CGAAAGC5500.046.1510243
CTGCCGC8450.042.032722145
CCGATAC750.001481316538.6789553
GTCCTAT8550.036.5334781
GACGACA1905.4405973E-934.335003145
GCGACGA850.002744776234.111716
ATTAGCC6350.031.962946
AGCACCG10000.031.172645
TAGCCCC6050.031.149568
GTACCGT1652.3816046E-630.767353
TACCGTC1652.3877037E-630.7572634
GCACCGT10450.030.5210026
TTATTCG1204.0639576E-430.202085145
ACCGTGT10100.030.1412938
GGGTTAC5100.029.9114691
GTATTAT14200.029.6707341
Produced by FastQC (version 0.11.5)