FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493971
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAACAGTGTGTTGCCACATTTAATTTGGGAGGGGGCGTTTAAAA15120.10120678379968555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4450.063.6012462
CCGACGC5000.060.885902145
GCCGACC1700.059.692062145
AGCGAAA5650.048.8347631
ATAGGCG450.00963995648.3334664
CGAAAGC5800.047.5112653
TCTATAC12850.034.4245033
CTGCCGC7400.033.3031145
CTATACC14750.029.4916064
TTATACG1000.00607532129.0000824
TATACCA15700.028.6296925
GTATACA7900.028.4923671
GTTCTAT14050.028.423681
GTATTAG5650.026.987631
GTATATA2456.3491825E-826.6729071
ATACCAC17500.025.6814866
TATTAGA6600.025.2897722
GTGTATA13500.024.2031921
AAGGCGC2151.8689152E-523.6007676
TATACTG5900.023.346745
Produced by FastQC (version 0.11.5)