FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905536
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9050.043.3304021
TGATCCG2550.042.638339
CGAAAGC9750.040.1698723
GCGAAAG10250.038.938412
GACGAAC2252.0008883E-1135.437187145
GACGCGC1458.7178705E-734.9928367
CGTAGAC2901.2732926E-1130.011973
ACGCGCC3700.029.3858788
TACGGGG1358.0950855E-426.8463577
GTCTACG1701.0203797E-425.6300751
TAAGACG2001.0656517E-525.3764654
TATACTG11950.024.2666345
CGTATTC1500.001496551624.161726
CGCGCCA3652.2009772E-1023.8307369
TCGTTAT2353.6649682E-521.6310561
TAGACAG18700.021.3225215
GTATAAG5850.021.1028271
CTAAACG1750.00365870420.7154834
GCCGACC3508.765528E-820.710047145
TGTACCG2505.935763E-520.3187712
Produced by FastQC (version 0.11.5)