FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760647
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7500.043.5735441
GCGAAAG8750.037.3190232
CGAAAGC9700.034.395533
GTATAGG5700.030.5779271
CTATTCG1902.0815241E-730.5206159
CGGATAG1703.0178217E-629.8473649
GCGTAAC1855.743501E-627.4788111
GACGGAT3251.4897523E-924.5338767
GCCGACC2751.9739491E-723.722841145
GTGATCG1851.8337579E-423.5091199
TATACCG2603.206127E-622.3079595
ATGTCGC2603.211664E-622.303524145
GTCCTAT13150.021.5382541
TAGTACT4151.0932126E-920.9658934
GGGTCGA4201.2732926E-920.7104159
GTATTAT13000.020.6695021
TACTGCG1800.00431301420.1351267
TAATACA17950.019.793014
TGATCCG6300.019.5598379
ATAATAC15250.019.0241853
Produced by FastQC (version 0.11.5)