FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480642
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCC7450.049.6221858
CGCGCCA7300.048.6558659
GCGAAAG7000.044.578242
GACGCGC4900.044.3799867
AGCGAAA7350.042.4923361
AACGCGC3600.040.270737
ATACGAC1303.6941128E-739.048163
CTGCCGC9500.037.38819145
GCCGACC3400.034.111675145
TACGACT1551.4654015E-632.7441254
TAAGACG7150.031.4356674
TCGTATA2951.4551915E-1129.5196842
GGGTTAC12600.029.3987671
GTCCTAT19300.028.977631
AAACGCG4800.028.6934726
AGACGCG7650.028.426976
AAGACGC8500.027.2920915
CGAAAGC12250.026.6392693
TATACGA2559.4107236E-825.6126692
TCCTATG24800.025.457752
Produced by FastQC (version 0.11.5)