FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2242_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2242_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632215
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC23910.14648805457614344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5400.044.344262
CGCGCCA4750.044.2570429
ACGCGCC4750.044.2570428
AGCGAAA6500.037.992321
CGAATAT2002.237357E-1036.24499145
TGATCCG4450.035.8377469
GCCGACC2850.035.60911145
AACGCGC2653.6379788E-1232.8256537
CTGCCGC8700.031.662292145
GACGCGC4150.031.4414397
CGAAAGC7800.030.6801193
AAACGCG2701.72804E-1029.5329576
AGACGCG4450.029.3217936
GTTATAC2654.5511115E-927.364773
CGAATAC2859.918949E-925.435081145
TATATAC4850.025.4182053
TACCGTA1801.5222866E-424.1633267
GTCCTAT13550.023.5854231
CTATCGC1851.833073E-423.5102639
TAGTAGT4455.456968E-1222.8079344
Produced by FastQC (version 0.11.5)