FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965598
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT33990.17292447387512605No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG22130.11258660214346983No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21360.10866921924014981No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT19780.10063095302294772No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC951.899678E-645.8666531
GCCGACC2500.040.59291145
GTGATCG2257.9489837E-1032.2165959
GCGAAAG4150.031.4707152
GTTGACA16900.031.3693541
CGAAAGC4500.030.6190073
GTATTAT30150.030.108711
GTTACGT1702.9743715E-629.9032611
ACATGGT18200.028.2830685
AGCGAAA4700.027.812761
TATACTG8200.027.4086325
TGATCCG1907.1697505E-626.7058669
GACTTAA8850.026.2588771
GCGTTAA3900.026.0210979
GTACTGC9600.025.6778284
TGCGTTA4250.025.5837698
TATTATT30550.025.1754262
CTGCCGC6050.025.160896145
GACATGG21500.024.2797724
GACGACT1500.001494533124.1673684
Produced by FastQC (version 0.11.5)