FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587062
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC23870.15040370193477004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGC6750.033.28782145
TATACGG1153.164147E-431.519675
GCGAAAG4150.029.7324492
GTGATCG2405.2887117E-827.1805749
GTTGACA12450.025.6717721
AGCGAAA4650.024.9942191
CTTATAT7100.024.5541781
GTATTAT14550.024.4627691
GTATATA6550.024.3979821
CGAAAGC5250.023.4872323
TACGGGG1550.001811637423.3811427
CCTATAT3854.1836756E-1022.6408671
GATATAT8450.022.35061
TATAGTA3905.0386006E-1022.3062295
GGTCCTA20450.021.9748429
GAATAAC12850.021.4809271
GCCGACC2704.4574026E-621.476011145
ATATAGT7200.021.1457774
ACATGGT14450.021.0712815
GCTACTA3508.767711E-820.709019
Produced by FastQC (version 0.11.5)