FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951320
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7850.052.7613871
GCGAAAG8650.048.6817442
CGAAAGC10300.040.1330073
TGATCCG4950.039.538699
GCCGACC2151.8153514E-830.343647145
TAGGGCG1306.4887514E-427.8819875
TACCGTC2001.067562E-525.3706597
CGCGCCA3201.2460077E-924.9176129
ATTACCG1500.001495601524.164395
ACGCGCC3601.8553692E-1024.1625338
GTGATCC15250.022.815778
GTCCTAT13600.021.3713591
ATAGTGA11950.021.232315
CGACTAT1750.003662502220.7118036
GACGCGC3851.05483196E-820.7107437
TATTAAT26600.019.6519242
GTACTGC6051.8189894E-1219.1712576
CGTCTAA1900.00581410819.121741
AGCACCG8350.019.0999985
GCACCGT8750.019.054866
Produced by FastQC (version 0.11.5)