FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373646
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT17380.1265245922166264No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG16530.12033668062950717No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT15390.11203759920678252No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA14410.10490330114163329No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC14030.10213694066739175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4600.058.3595662
AGCGAAA4650.057.780461
TGATCCG5300.045.138029
CGAAAGC6650.040.3513833
GGTCGTT1301.656106E-533.458958
ATAGTGA8100.033.1171345
AGTGATC8500.030.7035087
CGCGCCA4450.027.6944879
CATAGTG9300.027.2858164
ACGCGCC4550.027.0858178
TAGTACT3555.456968E-1226.5501424
TAGTGAT10250.025.4614476
ACGCTAA2001.0527125E-525.4155961
GCCGACC2001.066486E-525.373037145
GTATTAA13050.025.0399971
GTGATCC12200.024.9570878
TCATAGT11150.024.0660723
GACGCGC5150.023.930197
GTAGGAC5650.023.1048093
CCTTGTA4154.3655746E-1122.7281152
Produced by FastQC (version 0.11.5)