FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019097
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA13430.13178333367677464No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12470.12236322940799552No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT11920.11696629467067414No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG11530.11313937731148262No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11450.1123543686224177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1007.075869E-1058.1117171
CGACGAA657.3452154E-444.604816145
CGCGCCA750.001485127638.6575059
CAGTGCA7700.038.647852
ACGCGCC800.002039003236.241418
GTTGACA10100.034.5218121
GTATATC2309.822543E-1031.5824581
TTACGTG1851.6273407E-731.3870472
GATAGCG3950.031.19514145
CCGACTA3750.030.9351144
ACATGGT11900.029.2439465
AGTGCAG9950.029.1574233
GCTACTA2502.4538167E-928.9931329
CTTATAT4600.028.4242121
TATTGGG6000.027.8233072
CAGCAAC7300.027.8016327
GTAACAG4250.027.3466911
TAGCATG13850.027.251212
TATATGG4800.027.1984083
TATGGAC4550.027.0881425
Produced by FastQC (version 0.11.5)