FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655100
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17200.10392121321974504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1950.059.575171
TTACGTG2300.050.4834142
GCCGACC2252.0008883E-1135.440037145
GCTACTA3000.033.829139
TACGTGT3450.033.6332323
ACGTGTT4200.029.3451354
CAGTGCA12800.027.7806682
GTTATGT9800.027.4129351
TTAGGTA4300.026.9766484
GTTGACA16400.026.5636231
ACTACGC1100.00960393726.4026321
AGCGAAA3900.026.0641371
ACATGGT16500.025.9225855
GTACTAG2601.1294287E-725.1332741
ACTCCGA5200.025.1332741
CCGAGGT5800.024.999914
AGTGCAG14450.023.5883583
GGTTATA6050.022.8022731
CTGCCGC7000.022.782879145
GTATATC4850.022.4558451
Produced by FastQC (version 0.11.5)