FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997385
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6100.044.021542
AGCGAAA7550.035.5947271
CGAAAGC8400.031.9448033
CGCTATA1000.006044844629.0304222
TATAGCG1000.006044844629.0304222
CGTAGAC1801.517708E-424.1744483
GCTTACG1650.002574325522.0098421
ATCGTCG2003.1072416E-421.74728
CTGCCGC8350.020.83616145
TCGTCGA2155.0629565E-420.2299549
ATAGTCG2155.0629565E-420.2299548
TATACTG12100.019.772165
GTATAAG7950.019.185941
GTATAAT9900.018.3415361
ACTGATC11950.018.1984928
TAGACAG17600.018.1244815
GTCCTAT18550.018.0112881
GATAGCG3002.4203048E-416.914911145
TTATACT14600.016.8843464
GTATTAT22500.016.7861751
Produced by FastQC (version 0.11.5)