FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468742
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA259.3961623E-486.98011145
AGCGAAA4600.033.16931
CTACGGA1102.4312112E-432.9672133
GCGAAAG4500.032.2609832
ATATACG1204.049834E-430.2199463
GCCGACC1453.4969533E-529.99314145
GTTACGT2053.965779E-728.353781
CTGCCGC5400.028.187996145
TGACGCG1050.007718216627.612732145
AAGGTCG1606.840924E-527.1812829
CGAAAGC5350.027.113223
TTACGTG2259.017331E-725.8087862
GGTGCGA1450.001228353824.9942828
GCGGTGC1500.00149659824.1611398
TATACTG9050.024.0301075
CCGAGGT4600.023.6431434
GCACCGT7950.021.8832786
GTACCTA8000.021.7465066
TAGTCGG2353.7172547E-521.5908058
TAGTACC8400.021.579064
Produced by FastQC (version 0.11.5)