FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182676
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC27630.1265877299241848No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC27520.12608376140114244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAC2750.039.54127145
GCGAAAG5850.038.4526142
AGCGAAA6050.037.2198561
ACCGTCG800.002038200436.2461668
GGTTATA10500.033.2062871
GTATTAT23800.032.656921
TATACTG13400.029.2160035
ACGCGCC1000.006079152728.9969318
CGAAAGC8000.028.1023643
CGTATAC1050.007698888427.6275183
CGAAACG1050.00771008827.619294
GCACCGT7100.026.5470946
GAATAAC20450.024.8641221
AGTCGAA2101.5455984E-524.1879352
GTTATAT12650.024.092332
GTATTAG7300.023.8812371
GATAGCG3052.0398147E-823.767975145
TAGTACC9300.023.3873024
CTGCCGC6350.022.832228145
AGCACCG7100.022.464475
Produced by FastQC (version 0.11.5)