FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566890
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC23280.14857456490245008No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT16250.10370862026051605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4450.044.0734861
GCGAAAG4650.039.014892
TGACGCG800.002038809736.24332145
CGAAAGC5300.035.579923
GTTATAG3100.035.1482121
GCCGACC1652.3910015E-630.75191145
GTATTAT14700.030.1429751
TTATACT14350.028.7999554
TATACTG15050.028.420325
TTACGTG1804.6681726E-628.220772
AAGGTCG3351.8189894E-1228.1327365
GGTCGTT3205.456968E-1227.1824917
CCTTATA15000.027.091942
GTTGACA9750.026.8207871
TATATAG9100.026.3014743
GTTACGT1958.628776E-626.0757661
GAGCGAA1701.018255E-425.6375181
TGGCGAT2601.147655E-725.091532145
GTGATCG2901.1941665E-824.9953949
GGTTATA5950.024.4166831
Produced by FastQC (version 0.11.5)