FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078769
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6900.043.1270372
AGCGAAA7800.038.1830251
GACGCGC900.00363099732.218837
CGAAAGC9350.031.7972743
GTTAACG1050.007637635327.6727141
CCGGTAA3950.025.693497145
TATACTG10100.022.9689835
TACCGTA1600.00217726722.6538627
TTATACT9800.022.1936744
TATACTA5650.021.8129125
GTATAAT7500.021.3079911
ATAGTCG2053.6708883E-421.2172788
GTCCTAT17550.020.6954061
TCCTATG16400.020.3577062
GCACCGT10000.020.2978616
ATAATAC15050.020.2362693
GTATTAA23200.020.0388621
GTATAAG6900.020.002561
TATAAGC8850.019.6826172
TATAATA13250.019.1719842
Produced by FastQC (version 0.11.5)