FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2241_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2241_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563485
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6250.046.4830441
GCGAAAG6400.045.3514562
TATAGCG1953.6379788E-1240.9325942
CGAAAGC7700.037.6669853
GCGGTAT2252.8210707E-828.998016
TATACGC1306.490838E-427.8800289
AGCGGTA2802.3452048E-723.302725
CGGTATC2802.348097E-723.2997387
ATAGCGG2903.2846037E-722.5027753
ATATACG1650.002603995421.9660828
GTATTAT11250.021.9503271
GTATTAA11650.021.8200991
GCCGACC5001.8189894E-1221.746422145
CTGCCGC8350.021.703016145
CCTTATA9050.021.6484282
CGACCGC1700.003096518821.32002145
GTATTAG4601.72804E-1020.5258031
TAGCGGT3551.0160329E-720.421794
TATACTG10350.019.612815
TATACTA5851.0913936E-1118.6057262
Produced by FastQC (version 0.11.5)