FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2240_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2240_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480168
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC19930.1346468779219656No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG18990.12829624745299184No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC18910.12775576826414298No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG16800.113500629658255No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT16140.10904167635025212No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA14940.10093448851751963No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1701.7971615E-938.4554671
CAGTGCA7400.038.2563442
CATTCTA18350.036.7889562
TATACTG7150.035.4862445
GCGATTA1251.2660023E-534.797974145
CTACTGA24800.034.200486
GCGAAAG4900.032.590962
AGCGAAA4950.032.28361
GTATATC3000.031.4765111
TTGATTA9300.031.180979145
GTTGACA13850.030.9433041
TACTGAA27650.030.9374757
CCGACTA5000.030.4502834
CGAAAGC5350.029.8254533
TATTGGG10000.029.0355832
TTACGTG2252.7856004E-829.0355822
TAGCATG19800.027.8624292
AGTGCAG10450.027.7627093
CAAGCGG1606.811052E-527.198783
GACTTAA5100.027.0612531
Produced by FastQC (version 0.11.5)