FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2240_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2240_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423257
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC15330.10771069455481337No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14980.10525154627730621No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC14420.10131690903329477No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG300.001937286972.48702145
AGCGAAA3150.046.11911
GTTACGT1950.044.7000471
GGTGCGA1201.977005E-742.2840968
GATAGCG3950.040.372517145
TTACGTG2200.039.598192
AGACGAC2057.2759576E-1238.915983
TATACTG8250.037.789085
GACGACT1951.70985E-1037.1806684
AAGACGA2201.6370905E-1136.2983442
GTTGACA11650.034.915921
GCGAAAG4200.034.5698472
CGAAAGC4500.032.2334373
ACATGGT12600.031.6478315
ACGTGTT2755.456968E-1231.637374
CGACTCC2755.456968E-1231.6329216
ACTCCGA5750.031.5815541
CCTTATA6450.031.514842
CCGAGGT6000.031.4176654
AAGGTCG1652.3907014E-630.7520719
Produced by FastQC (version 0.11.5)